The gut is one of the primary sites for exposure with chemicals and hence a vulnerable target for toxic effects. We hypothesize that intestinal exposure to such chemicals can trigger gut inflammation. Macrophages, key innate immune cells, represent a promising readout for detecting immunomodulatory effects following chemical exposure. To investigate this hypothesis, we developed an open-source...
Selective plane illumination microscopy (SPIM, also called light-sheet fluorescence mi-croscopy) is the method of choice for studying organ morphogenesis and function as it permits gentle and rapid volumetric imaging of biological specimens over days. In inho-mogeneous samples, however, sample-induced aberrations, including absorption, scat-tering, and refraction, degrade the image,...
Automated multi-perspective imaging of zebrafish larvae allows researchers to accelerate analysis without the arduous task of repositioning specimens under a microscope. Systems, such as the VAST BioImager facilitate this, but they are expensive and lack adaptability for specific laboratory needs. Additionally, the glass capillary size used (600-700 µm) restricts the size of the larvae, which...
