The gut is one of the primary sites for exposure with chemicals and hence a vulnerable target for toxic effects. We hypothesize that intestinal exposure to such chemicals can trigger gut inflammation. Macrophages, key innate immune cells, represent a promising readout for detecting immunomodulatory effects following chemical exposure. To investigate this hypothesis, we developed an open-source...
Selective plane illumination microscopy (SPIM, also called light-sheet fluorescence mi-croscopy) is the method of choice for studying organ morphogenesis and function as it permits gentle and rapid volumetric imaging of biological specimens over days. In inho-mogeneous samples, however, sample-induced aberrations, including absorption, scat-tering, and refraction, degrade the image,...
Automated multi-perspective imaging of zebrafish larvae allows researchers to accelerate analysis without the arduous task of repositioning specimens under a microscope. Systems, such as the VAST BioImager facilitate this, but they are expensive and lack adaptability for specific laboratory needs. Additionally, the glass capillary size used (600-700 µm) restricts the size of the larvae, which...
Machine Learning-based phenotype classification of zebrafish embryos enables fast and reproducible assessments for toxicity evaluation. However, acquiring large amounts of labeled data can be expensive, time-consuming and requires biological expertise. Based on the publicly available EmbryoNet dataset, we explore the impact that less available labeled data has on the performance of machine...
Zebrafish embryos are frequently used as an experimental model in the assessment of toxic effects. They are considered as alternatives to animal testing, since early life stages are not considered as “protected” stages by recent European regulations on animal welfare. An important read-out in effect assessment of chemicals in the zebrafish embryo is the assessment of morphological alterations....
