4–6 Oct 2023
Biozentrum, University of Würzburg
Europe/Berlin timezone

Chlamydomonas Reinhardtii 3D Motion Captured by Holographic Microscopy

Not scheduled
20m
Room A101 (Biozentrum, University of Würzburg)

Room A101

Biozentrum, University of Würzburg

Am Hubland
Poster

Speaker

Mr Nicolas Fares (Univ. Bordeaux, CNRS, LOMA, UMR 5798)

Description

Motility of microscopic entities is a central question in biology. In an idealization attempt, the problem could be reduced to four key elements: viscous flow, soft confinement, thermal fluctuations and activity.
To address this matter, a novel method based on Mie holography and stochastic inference was developed in the group [1]. In a nutshell, this method allows to track particles in 3 dimensions, with a resolution of 10 nm. Over the past years, it led to quantify experimentally and theoretically how a Brownian sphere is affected by complex confined situations, such as rigid and charged walls, elastomeric ones or liquid interfaces. Specifically, in the case of a sphere diffusing in salted walter on top of a glass wall, fine deviations from the bulk Gaussian statistics of displacements were quantified theoretically and numerically and measured experimentally [2]. Also, surface forces are measured down to a few femtonewtons. Thus, the method is a promising contactless and gentle probe, since it is only driven by thermal fluctuations.
After that robust calibration of the technique, more complex situations can be studied. The first one is the motion ofthe active alga Chlamydomonas reinhardtii. Its 3D motion was successfully tracked, which paves the way for tackling questions concerning the alga's behavior in confinement, or even gliding properties.

References
[1] Maxime Lavaud, Thomas Salez, Yann Louyer, and Yacine Amarouchene. Stochastic inference of surface-induced effects using Brownian motion. Physical Review Research, 3(3):L032011, July 2021.
[2] Arthur Alexandre, Maxime Lavaud, Nicolas Fares \textit{et al.}. Non-Gaussian diffusion near surfaces. Physical Review Letters, 130(7), 077101.

Primary authors

Mr Nicolas Fares (Univ. Bordeaux, CNRS, LOMA, UMR 5798) Mr Maxime Lavaud (Univ. Bordeaux, CNRS, LOMA, UMR 5798) Mr Antoine Allard (Univ. Bordeaux, CNRS, LOMA, UMR 5798) Prof. Oliver Bäumchen (Experimental Physics V, University of Bayreuth, Germany) Mr Yacine Amarouchene (Univ. Bordeaux, CNRS, LOMA, UMR 5798) Mr Thomas Salez (Univ. Bordeaux, CNRS, LOMA, UMR 5798)

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