Imaging - From Organisms to Molecules
from
Thursday, April 28, 2022 (2:00 PM)
to
Thursday, July 14, 2022 (3:30 PM)
Monday, April 25, 2022
Tuesday, April 26, 2022
Wednesday, April 27, 2022
Thursday, April 28, 2022
2:00 PM
Imaging Physics in Medicine
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Mark Ladd
(Medical Physics in Radiology, German Cancer Research Center (DKFZ))
Imaging Physics in Medicine
Mark Ladd
(Medical Physics in Radiology, German Cancer Research Center (DKFZ))
2:00 PM - 3:30 PM
Macroscopic imaging in medicine is used both for clinical diagnostics and therapy planning in human patients as well as in research, where both human and small-animal imaging play an important role. Medical imaging is based on several physical principles ranging from ultrasound to ionizing radiation coming from sources outside or inside the patient. Accordingly, the questions to be answered by the different methods and the information in the resulting images are quite different. In this lecture, the physical and technical principles of techniques such as computer tomography (CT), positron emission tomography (PET), and magnetic resonance imaging (MRI) will be introduced.
Friday, April 29, 2022
Saturday, April 30, 2022
Sunday, May 1, 2022
Monday, May 2, 2022
Tuesday, May 3, 2022
Wednesday, May 4, 2022
Thursday, May 5, 2022
Friday, May 6, 2022
Saturday, May 7, 2022
Sunday, May 8, 2022
Monday, May 9, 2022
Tuesday, May 10, 2022
Wednesday, May 11, 2022
Thursday, May 12, 2022
2:00 PM
Analyzing Medical Images Using Machine Learning
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Paul Jäger
(Helmholtz Imaging, DKFZ)
Lukas Klein
(Helmholtz Imaging, DKFZ)
Analyzing Medical Images Using Machine Learning
Paul Jäger
(Helmholtz Imaging, DKFZ)
Lukas Klein
(Helmholtz Imaging, DKFZ)
2:00 PM - 3:30 PM
The success of Machine Learning has revolutionized the field of medical image analysis in the past 5 years. This talk will give an introduction to relevant concepts in machine learning with a focus on computer vision. Subsequently, several example applications in the biomedical domain will be discussed to study the current state of research and the associated challenges and opportunities.
Friday, May 13, 2022
Saturday, May 14, 2022
Sunday, May 15, 2022
Monday, May 16, 2022
Tuesday, May 17, 2022
Wednesday, May 18, 2022
Thursday, May 19, 2022
Friday, May 20, 2022
Saturday, May 21, 2022
Sunday, May 22, 2022
Monday, May 23, 2022
Tuesday, May 24, 2022
Wednesday, May 25, 2022
Thursday, May 26, 2022
Friday, May 27, 2022
Saturday, May 28, 2022
Sunday, May 29, 2022
Monday, May 30, 2022
Tuesday, May 31, 2022
Wednesday, June 1, 2022
Thursday, June 2, 2022
2:00 PM
Optical Coherence Tomography in Biofilm Research - Visualization and Characterization of the Mesoscopic Biofilm Structure
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Michael Wagner
(KIT)
Optical Coherence Tomography in Biofilm Research - Visualization and Characterization of the Mesoscopic Biofilm Structure
Michael Wagner
(KIT)
2:00 PM - 3:30 PM
Understanding the biofilm structure is important in order to understand the structure-function relations of biofilm systems. For decades microscopic tools have widely been applied visualizing the microscopic structure of biofilms (e.g., distribution of cells within the matrix of extracellular polymeric substance). However, mass transport and transfer occurs at a larger scale (mesoscale, mm-scale). By applying optical coherence tomography (OCT) the mesoscopic biofilm structure is assessed non-invasively, in situ and with almost no sample preparation. OCT thus has become popular in biofilm research within the last 10 years. This lecture will introduce OCT and provide insights into the technology and possible outputs (e.g., structure analysis).
Friday, June 3, 2022
Saturday, June 4, 2022
Sunday, June 5, 2022
Monday, June 6, 2022
Tuesday, June 7, 2022
Wednesday, June 8, 2022
Thursday, June 9, 2022
Friday, June 10, 2022
Saturday, June 11, 2022
Sunday, June 12, 2022
Monday, June 13, 2022
Tuesday, June 14, 2022
Wednesday, June 15, 2022
Thursday, June 16, 2022
Friday, June 17, 2022
Saturday, June 18, 2022
Sunday, June 19, 2022
Monday, June 20, 2022
Tuesday, June 21, 2022
Wednesday, June 22, 2022
Thursday, June 23, 2022
2:00 PM
Basics of Light Microscopy for the Study of Cells
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Rudolf Merkel
(FZJ)
Basics of Light Microscopy for the Study of Cells
Rudolf Merkel
(FZJ)
2:00 PM - 3:30 PM
Light microscopy is one of the most important techniques for the investigation of animal cells. In this lecture I will present basics of widely used light microscopy techniques including fluorescence and confocal fluorescence microscopy as well as their application on living cells.
Friday, June 24, 2022
Saturday, June 25, 2022
Sunday, June 26, 2022
Monday, June 27, 2022
Tuesday, June 28, 2022
Wednesday, June 29, 2022
Thursday, June 30, 2022
2:00 PM
Advanced Fluorescence Microscopy
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Gerd Ulrich Nienhaus
(Institute of Applied Physics, KIT)
Advanced Fluorescence Microscopy
Gerd Ulrich Nienhaus
(Institute of Applied Physics, KIT)
2:00 PM - 3:30 PM
After a brief, general introduction into fluorescence microscopy, I will focus on three modern modalities, STimulated Emission Depletion (STED) nanoscopy, Single-Molecule Localization Microscopy (SMLM) and Light Sheet Microscopy (LSM). STED and SMLM are powerful techniques for cellular imaging with nanoscale spatial resolution, whereas LSM is an excellent and versatile imaging method with diffraction-limited resolution and low phototoxicity. Pros and cons of the methods for particular imaging applications will be discussed.
Friday, July 1, 2022
Saturday, July 2, 2022
Sunday, July 3, 2022
Monday, July 4, 2022
Tuesday, July 5, 2022
Wednesday, July 6, 2022
Thursday, July 7, 2022
2:00 PM
Microscopy Assessment of DNA-based Information Processing in Biological and Artificial Systems
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Lennart Hilbert
(KIT)
Microscopy Assessment of DNA-based Information Processing in Biological and Artificial Systems
Lennart Hilbert
(KIT)
2:00 PM - 3:30 PM
The processing of DNA sequence information is a central task of biological cells, and could in the future enable molecular biotechnologies with “smart capabilities”. In our research group, we combine cutting-edge light microscopy, computational data analysis, and simulations to understand the principles of this information processing in the 3D-space of the cell nucleus. In this lecture, I will outline how such imaging-informed assessments can reveal major principles of DNA-based information processing, and how these principles might in the future guide the design of artificial, DNA-based information systems.
Friday, July 8, 2022
Saturday, July 9, 2022
Sunday, July 10, 2022
Monday, July 11, 2022
Tuesday, July 12, 2022
Wednesday, July 13, 2022
Thursday, July 14, 2022
2:00 PM
Imaging Biological Molecules by Electron Cryo-Microscopy (cryo-EM)
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Carsten Sachse
(Forschungszentrum Jülich GmbH, Ernst-Ruska Centre for Microscopy and Spectroscopy with Electrons / ER-C-3 Structural Biology, Institute for Biological Information Processing / IBI-6 Cellular Structural Biology)
Imaging Biological Molecules by Electron Cryo-Microscopy (cryo-EM)
Carsten Sachse
(Forschungszentrum Jülich GmbH, Ernst-Ruska Centre for Microscopy and Spectroscopy with Electrons / ER-C-3 Structural Biology, Institute for Biological Information Processing / IBI-6 Cellular Structural Biology)
2:00 PM - 3:30 PM
The cryo-EM method has become very powerful in the past several years and it is becoming the method of choice for many structural biologists. The interpretation of single noisy molecular images in solution still presents an obstacle for obtaining faithful structural information of biological macromolecules. Therefore, we routinely apply image processing routines of structural averaging in single-particle cryo-EM as well as cryo-tomography. Small quantities of purified proteins and protein complexes can be visualized in vitreous ice using single-particle cryo-EM. Subsequent data processing of 1000s of molecular images enables three-dimensional structure determination at near-atomic resolution. The obtained atomic models provide the chemical framework for biological macromolecules with respect to their biological function. Alternatively, more complex biological environments such as cells are increasingly being visualized by cryo-EM methods. Electron cryo-tomography combined with fluorescence light microscopy enables the detailed characterization of natively preserved cells, the organelle ultrastructures and provides the link to their molecular interpretation. In the talk, we will explain the physical foundations of the cryo-EM techniques and associated image processing approaches and combine them with recently developed applications that assist in the interpretation of the native electron cryo-micrographs.