Filippo Pisano
(Department of Physics and Astronomy, University of Padova, Padova, Italy & Padova Neuroscience Center, University of Padova, Padova, Italy)
Optical approaches for in vivo neural monitoring offer a precious window on brain functions and on the mechanisms of development, ageing or disease progression. Nonetheless, the existing methods still struggle to capture in situ the complex biomolecular alterations that accompany physiological and pathological dynamics. As a result, our grasp on the multifaceted components of brain activity is still limited.
To surpass this limitation, we propose a vibrational fiber photometry method based on spontaneous Raman scattering that allows monitoring the biomolecular content of arbitrarily deep brain volumes of the mouse brain in vivo without exogenous reporters. To do this, we employed a single, thin tapered optical fiber delivering and collecting optical signals to gather information on the local cytoarchitecture, to sense molecular alterations linked to circuit dysfunction caused by traumatic brain injury, and to detect diagnostic markers of brain metastasis with high accuracy.
In our view, vibrational fiber photometry offers an opportunity to capture a more comprehensive picture of neural activity in the biomolecular context of the local micro-environment. This capability, that can be employed alongside traditional fiber photometry or electrophysiological techniques, is particularly promising for empowering emerging research on brain-immune [1] and brain-cancer [2] bidirectional dynamics.
References
[1] Castellani, G. et al., Science (1979) 380, (2023).
[2] Mancusi, R. & Monje, M., Nature 618, 467–479 (2023).
Filippo Pisano
(Department of Physics and Astronomy, University of Padova, Padova, Italy & Padova Neuroscience Center, University of Padova, Padova, Italy)
Mariam Masmudi-Martín
(Brain Metastasis group, CNIO, Madrid, Spain)
Maria Samuela Andriani
(Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, Arnesano, Italy & Dipartimento di Ingegneria dell’Innovazione, Università del Salento, Lecce, Italy)
Elena Cid
(Instituto Cajal, CSIC, Madrid, Spain)
Mohammadrahim Kazemzadeh
(Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, Arnesano, Italy)
Marco Pisanello
(Optogenix srl, Lecce, Italy)
Antonio Balena
(Department of Physics and Astronomy, University of Padova, Padova, Italy)
Liam Collard
(Department of Physics and Astronomy, University of Padova, Padova, Italy)
Teresa Jurado Parras
(Dipartimento di Ingegneria dell’Innovazione, Università del Salento, Lecce, Italy)
Marco Bianco
(Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, Arnesano, Italy)
Patricia Baena
(Brain Metastasis group, CNIO, Madrid, Spain)
Francesco Tantussi
(Istituto Italiano di Tecnologia, Center for Convergent Technologies, Genova, Italy)
Marco Grande
(Dipartimento di Ingegneria Elettrica e dell’Informazione, Bari, Italy)
Leonardo Sileo
(Optogenix srl, Lecce, Italy)
Francesco Gentile
(Nanotechnology Research Center, Department of Experimental and Clinical Medicine, University of “Magna Graecia”, Catanzaro, Italy)
Francesco De Angelis
(Istituto Italiano di Tecnologia, Center for Convergent Technologies, Genova, Italy)
Massimo De Vittorio
(Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, Arnesano, Italy)
Liset Menendez de La Prida
(Instituto Cajal, CSIC, Madrid, Spain)
Manuel Valiente
(Brain Metastasis group, CNIO, Madrid, Spain)
Ferruccio Pisanello
(Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, Arnesano, Italy)
There are no materials yet.
